The
Halobacterium
sp. NRC-1
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orc7
Gene ID
2411
Gene Name
orc7
Annotation
orc / cell division control protein 6
Location
chromosome:1807230→1808783
Gene type
Functional gene
GC content
63.39
Hydropathicity
-0.476640926640926
DNA (1554 bp,
Edit
)
>orc7|2411|1807230|For|1808783
atgacagacgatcggtcgacggacgaacggcgaaccgcggaccgcgacttcgaagtcgacctcgacgacgtcctcgacga
cgaggacgacgacgagggactcttcgatgacctgctcagcggcgagcccatcttcgagaacaaagaggtcctgcggccgt
cgtacacgccccacgaactcccacaccgaaacgaccagatcaacaacatggcgaccatcctggtcgcggcgctccgcggt
gagacgccgtcgaacatcctcatctacgggaagacgggcaccggaaagacggccagcgcgaagttcgtgagccaggagtt
ggagaagacctccaagaagtacgacgttccctgtgaggtcgaatacatcaactgcgaagtcacggacacccagtaccggg
tgctcgcacagctcgccaacacgttcatcgatcaaaaccgggcgttcgtcgacgcccgcatcgccgacctctcggacctc
cgggcacaggccgacgacgaccccaacgcactcgaaataggggggtcgccgggcgacgatcgtacaggcaacgaatcgag
tgagggctccgatgtttcggacagcttttcggatcttcgggagcgcttcgactccgtcgcggagatcgacgaccgcatcg
actccctggaagccgacaaggccgagatggaacaggtccccatgacagggtggcccaccgaccgcgtgtacgcgacgttc
ttcgacgccgtcgactacgtcgagcgcgtcgcggtcatcatgctggacgagatcgacaagctcgtcgagaaatccggcga
cgatacgctgtacaacctctcccgcatgaactccgagttggacaactcccgggtgtcgatcatcggcatcagcaacgacc
tgaagttcaccgacttcctcgacccccgtgtcaagtccagcctcggcgaggaggagatcgtgttcccgccgtacgacgcc
aaccagctgcgggacatcctccagcaccggtcggacgtcgcgttcaaagccgacgcgctctcggacgacgtcctcccgtt
gtgtgcggcgttcgccgctcaggagcacggcgacgcccgtcgcgcccttgacctgctgcggaccgccggcgagctcgcgg
agcgcgaccaggccgaaaacgtcaccgaggaccacgtgcgccgcgcccaagacaagatcgagctcgaccgcgtcgtggag
gtcgtccgaacgctgccgacacagagcaagctcgtgctctacgccattctcctgttggaggacaacggcgtgcacaacgt
caacacgggtgaggtgtacaacatctacaagacgctgtgcgacgagctcgacgccgacgtgctcacgcagcgccgggtca
ccgacctcatcagcgaactcgacatgctcggcatcgtcaacgccgtcgtcgtctcgaagggccggtacggccgcaccaaa
gagatctcgctgtcggttccggtcgaggagacggaggcggtcctcgaagcggattcgcggctcagcgacatcgaagacat
cacgccgttcgtgcaggcgcgcttcgagaacaattag
pI
4.1
MW (k)
58.05435
Translation (518 aa,
Edit
)
>orc7|2411|gene
MTDDRSTDERRTADRDFEVDLDDVLDDEDDDEGLFDDLLSGEPIFENKEVLRPSYTPHELPHRNDQINNMATILVAALRG
ETPSNILIYGKTGTGKTASAKFVSQELEKTSKKYDVPCEVEYINCEVTDTQYRVLAQLANTFIDQNRAFVDARIADLSDL
RAQADDDPNALEIGGSPGDDRTGNESSEGSDVSDSFSDLRERFDSVAEIDDRIDSLEADKAEMEQVPMTGWPTDRVYATF
FDAVDYVERVAVIMLDEIDKLVEKSGDDTLYNLSRMNSELDNSRVSIIGISNDLKFTDFLDPRVKSSLGEEEIVFPPYDA
NQLRDILQHRSDVAFKADALSDDVLPLCAAFAAQEHGDARRALDLLRTAGELAERDQAENVTEDHVRRAQDKIELDRVVE
VVRTLPTQSKLVLYAILLLEDNGVHNVNTGEVYNIYKTLCDELDADVLTQRRVTDLISELDMLGIVNAVVVSKGRYGRTK
EISLSVPVEETEAVLEADSRLSDIEDITPFVQARFENN
Set Format
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tab-delimited
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Comment
The orc7 gene and upstream region endows autonomous replication ability on non-replication plasmids containing the selectable mevinolin resistance marker. The upstream region contains a nearly perfect inverted repeat of 33 bp flanking an extremely AT-rich stretch of 189 bp (56% GC). One of two chromosomal inflection points occur here in cumulative GC-skew analysis used for prediction of replication origins. See Berquist and DasSarma [J Bacteriol. 2003 Oct;185(20):5959-66](https://www.ncbi.nlm.nih.gov/pubmed/14526006) and Coker et al. [J Bacteriol. 2009 Aug;191(16):5253-61](https://www.ncbi.nlm.nih.gov/pubmed/19502403). However, orc7 was not found to be essential based on the ability to delete the gene. See Berquist et al. [BMC Genetics 2007 June 8;8:31](https://www.ncbi.nlm.nih.gov/pubmed/17559652).
