The
Halobacterium
sp. NRC-1
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pyrF
Gene ID
1673
Gene Name
pyrF
Annotation
orotidine-5'-monophosphate (Ura3)
Location
chromosome:1246366→1247166
Gene type
Functional gene
GC content
71.79
Hydropathicity
-0.257303370786517
DNA (801 bp,
Edit
)
>pyrF|1673|1246366|For|1247166
atgagcttcgtcgaggaactcggggcccgcatcgaggcggctgactcggtggtgagcgtgggtctcgatccggacatgga
gcggcttccggaggacgtacaggacgcggagctgccgcggtgggcgttcaaccgccgcatcatcgacgcgacccacgagc
acgccgcggtgttcaagccgaacgcggcgttctacgaggacagcgacgggtggcgcgcgctccgggagacggtggcgtac
gcccacggcaagggcgtgcccgtgttgttggacgcgaagcgcgcggacatcgggaacacggcccgccagtacgccgagat
cctggcgcacgtcgacgccatcaccgtcaacccgtacctcggggaggacgccctgcagccgttcctcacgcaggacgagg
cgggcgtgttcgtgttgtgtcgcacctccaacgagggcgggatggatttccagcatctcgaactcgcggcctacgaccgc
cggctctacgagcacgtcgccgagcgggcggccgagtggaacgcccagtacggggatgtcgggctggtggtgggcgcaac
cgcgcccgacgagctccaggccatccgggagcgcgtgccggagctgccgttcctggtgccgggcgtgggcgcgcagggcg
gcgacgccgaggccgccgtggagtacgggctcaacgacgacggcgtcgggctggtgaactcgacgcgcggcgtcatcttc
gcgggcgaacacggctcagcgtgggcggcggcggccggcgacgcggcgcggacgctgcgcgagcgcctgaaccgccaccg
gtag
E.C.#
4.1.1.23
pI
4.5
MW (k)
29.04796
Translation (267 aa,
Edit
)
>pyrF|1673|gene
MSFVEELGARIEAADSVVSVGLDPDMERLPEDVQDAELPRWAFNRRIIDATHEHAAVFKPNAAFYEDSDGWRALRETVAY
AHGKGVPVLLDAKRADIGNTARQYAEILAHVDAITVNPYLGEDALQPFLTQDEAGVFVLCRTSNEGGMDFQHLELAAYDR
RLYEHVAERAAEWNAQYGDVGLVVGATAPDELQAIRERVPELPFLVPGVGAQGGDAEAAVEYGLNDDGVGLVNSTRGVIF
AGEHGSAWAAAAGDAARTLRERLNRHR
Set Format
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tab-delimited
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Comment
pyrF (or ura3) has been developed as a selectable and counter selectable marker for 5-fluoroorotic acid, which permits construction of gene knockouts and replacements. See Peck RF, DasSarma S, Krebs MP. [Mol Microbiol. 2000 Feb;35(3):667-76.](https://www.ncbi.nlm.nih.gov/pubmed/10672188)