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ISH1, 1,118 bp long, was discovered when it inserted into the bop gene in bacteriorhodopsin deficient mutants of Halobacterium sp. S9. It integrated in both orientations into the N-terminal coding region of BR duplicating an 8 bp sequence which is flanked by short (9/10 bp) inverted repeats, which forms a stem-loop at the 5'-end of the bop mRNA. ISH1 codes an 810 bp long open reading frame which is transcribed into a 900 nucleotide long mRNA. See (Simsek, DasSarma, RajBhandary, and Khorana, Proc. NatL Acad. Sci. USA. Vol. 79, pp. 7268-7272, December 1982)[http://www.pnas.org/content/79/23/7268.short] for more details. |
