[R] (-) COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold 9 genomes BUD0086 10 proteins a Hsa Halobacterium sp. NRC-1 b Hma Haloarcula marismortui f Hvo Haloferax volcanii g Hbo Halogeometricum borinquense h Hmu Halomicrobium mukohataei i Hut Halorhabdus utahensis j Hla Halorubrum lacusprofundi q Nph Natronomonas pharaonis x Hwa Haloquadratum walsbyi VNG0851 rrnAC0400 rrnAC3033 HVO_1626 Hbo3218 Hmu1365 Hut0196 hla2022 NP3152A HQ2591A

Pairwise sequence scores
Consensus score	2201	rrnAC3033	VNG0851	hla2022	HQ2591A	HVO_1626	Hbo3218	NP3152A	Hut0196	rrnAC0400	Hmu1365
1330	rrnAC3033	2288	1147	1129	1144	1181	1168	1063	1209	1187	1195
1697	VNG0851	1147	2531	1434	1417	1540	1473	1453	1472	1412	1448
1712	hla2022	1129	1434	2244	1496	1586	1585	1423	1464	1410	1462
1674	HQ2591A	1144	1417	1496	2279	1571	1719	1447	1421	1452	1410
1775	HVO_1626	1181	1540	1586	1571	2198	1742	1429	1518	1568	1538
1763	Hbo3218	1168	1473	1585	1719	1742	2208	1460	1499	1531	1489
1662	NP3152A	1063	1453	1423	1447	1429	1460	2188	1467	1496	1524
1747	Hut0196	1209	1472	1464	1421	1518	1499	1467	2175	1535	1603
1761	rrnAC0400	1187	1412	1410	1452	1568	1531	1496	1535	2216	1790
1730	Hmu1365	1195	1448	1462	1410	1538	1489	1524	1603	1790	2211

332 symbols
consensus       -----------------------------------------MVDSDWGDWLPRA-VEAAD
#oth            1111111111111111111111111111111212333333413611.22.1121111213
rrnAC3033       :::::::::::::::::::::::::::::::::::::::::::Q:T:D::FV:DEI::T:
VNG0851         MRGDDIDSSAMTLITSYTCPRAVKCPGRTTERLGRGPPTAG::H::::::::A:::A:::
hla2022         ::::::::::::::::::::::::::::::::::MHSQESD:T::::S::::::::AD::
HQ2591A         :::::::::::::::::::::::::::::::MLDVYIDNMSTIKT:::S::::D:::::S
HVO_1626        ::::::::::::::::::::::::::::::::::::::::::TV:::S:::::::::S::
Hbo3218         ::::::::::::::::::::::::::::::::::::::::::SV:::::::::::::D:S
NP3152A         ::::::::::::::::::::::::::::::::::::::::MTIH::::::::T::IA::E
Hut0196         :::::::::::::::::::::::::::::::::::::::::::S::::E:::A::I:STT
rrnAC0400       ::::::::::::::::::::::::::::::::::::::::MT:E:::D:::::::::S:T
Hmu1365         ::::::::::::::::::::::::::::::::::::::::MT:::::::::V:::::D::

consensus       P-DGVAIWYLGCNGFVLKGSDGTTLFIDPYLGTGDPPRTVRMIPVPFDPRDVTEADAVLA
#oth            .121222.......1321323111231...2.2....111..1...12.6.2542..1.2
rrnAC3033       :G:::V:::::::::::R-:QS:::Y::::F::::::NLI::::::M::A:A:QC::T:V
VNG0851         ::::I:V:::::::VI::A:::::::V:::::::::::::::::::::::::SD::::::
hla2022         :::T::L::::::::AI:::E::V:W::::V::::::::::::::::::D:ID:::::::
HQ2591A         ::NT:::::::::::F:::NE:::IA::::V::::::::::::::::::G:I:TM:::::
HVO_1626        ::ET::::::::::::I:::E:::::V:::V:I::::::::::::::::E::::::::::
Hbo3218         :::T:S:::::::::F:::::::::A::::V:L::::::::::::::::H::A:M:::::
NP3152A         ::::L:V::::::::A::AA::::V::::::::::::::::::::::E:T::V:::::::
Hut0196         :::::::::::::::::::AG:::I:::::::::::::::::::::::::::RA::::::
rrnAC0400       ::::L::::::::::IV:S:G:::V:::::::::::::::::V::::N:E:I::C::::G
Hmu1365         ::::L:::::::::::::S:G:SVVYV:::::I::::::::::::::::D::R:::::::

consensus       THEHTDHVHGPSQAPILAGTGADFYAPDASLAVARDDEAWTDEYDVDDDQLREVTEGDTL
#oth            ..1.2..2.12.11.11241115222133143243351415233525332483133.124
rrnAC3033       ::::I::M:P::YG:LVEDL:::L:::S::Y----:SPDYDG:MR:P:E:RN:IAV::EF
VNG0851         :::::::::::::::L::D::CSL:::E::I:::::::R:Q:D:A:TT::Y:R:AV:::Y
hla2022         ::::::::::::::::::N:D:::V:::D::::::EE:R::E:WE::EEAFL::::::E:
HQ2591A         :::::::T:::::G:::ES:D:R:::::D:ID:::ET:::Q::::INET::QVIS::::I
HVO_1626        ::::::::::Q::::::EN:::T:V:::D::S::Y:::::F:D::LSE::FE::A:::SV
Hbo3218         :::::::::::::::::ES:::QY::::D::D::LE::::V:D::ISEE:FT:::D:::F
NP3152A         ::D::::T:::::::::T::D:T::G:PT:V:T:---::::::HG::GNDIA:I:::E::
Hut0196         ::D:V:::::R:::::::E:::E:F::::::::T-:A:G::::W::A::::SS:SI::EI
rrnAC0400       :::::::::::::::::::::::Y:TT:SGHD:I:E-:::LEN:S:T::::H::::::::
Hmu1365         :::::::T::::::::::::::T::GT:::HD:V-SA:N::ETW::::E::::IG::::F

consensus       EIGEFTVHVEPANDPDATHPVSYVIEHDAGTFFHGGDARPAE-FEAVGERYDIDLGVLAF
#oth            31.2111..3523.1.1521.2..21.12..21.1..21.42414421452.1.2.21..
rrnAC3033       AV:D:::::TGT:::::IE::G::::::::::::A::S::::A:SD:::QF:::V:A:::
VNG0851         D::S:::::T::H::::VQ:::::F::::::V:::::TK::DALAEI:AAH:::V:I:::
hla2022         K::::::::VETE:A::::::G::::::S::L::A::SK:SAS:P:LADEF:::::I:::
HQ2591A         D:::::::::Y:H:::S:::::::::::T::::::::TK:H:D:D:L:NA::::::::::
HVO_1626        D:::::::::::Y::::S:::A::F::ES:::I::::TK:::E:AGI:::::L:::I:::
Hbo3218         :V::::I:::HSY::::::::::::::ES::::::::TK:D:E:::L::K::::::::::
NP3152A         :V:SLRI:::::H::::D:::G::V::ES:::::::::::S:D:::I:GS::::::AV::
Hut0196         :V::::I::AD::::::EE::::::Q:E::::::::::::GA:::S::G:F:::::::::
rrnAC0400       :::DL::::::::::::E:S::::F:::::::::::::::G::::S::::::::A:::::
Hmu1365         :V:DL::::::::::::D::::::F:::::::::::::::GA:::S:::S::::V:::::

consensus       GSVGMIPDKETGEPVRTKWYNDENEIIEAANDLQLDRLLPTHWDMWKGLTADPTALHAHA
#oth            .13.412123231143.3..32.122231.33.322212.1.1...1.2121.11.24.2
rrnAC3033       :T::S:YEP:PDSG::::::M::::::::::Q:::S::::S:H:::Q:EAG::KV::E::
VNG0851         ::T:E::::Q:R::SV:T:::T:T:VVAC::A::::::V::::::::::::::::::::T
hla2022         ::::RV:::::::::E::::S:::::AA::E::A::::I::::::::::::::::::E:V
HQ2591A         ::I:N:D::D::::::::::AT:::::R::AA:NI:::::S:::::::::::::::::::
HVO_1626        :T::N::::::::::::T::::::Q:::::S::D::::V:::::::::M::::KV::H::
Hbo3218         ::I:N:D:::::::::::::ST:::AVK::S:::F::F::S:::::::::::::V::H::
NP3152A         ::A::::::::R::KY:Q::SN::MAA:::AQ::::::::::::::::::::::::RD:T
Hut0196         :::::V::AIGE:::::R::::::M:V::::E::::A:::S:::::::M:::::::YD:V
rrnAC0400       :T::::D:::::::T::Q::::::M::::::E::::T:I:::::::::M:TE::V::N::
Hmu1365         :T:::::::::R::E:::::::::M:V::::E:::ET:V:::::::::M:TE::V::N:V

consensus       RSFAHPRRLEIVEIGDRIDL------------
#oth            4.354.6522331..11333311111111111
rrnAC3033       V::DY::VI:PLY::CSVR:SEPGIRRLDALD
VNG0851         ::M:S:Q::DV::::::::VS:::::::::::
hla2022         ::RS::NQ::::::::::::::::::::::::
HQ2591A         Q:YTF::VI::A:::::::I::::::::::::
HVO_1626        K::EA::S:DL::::::V::::::::::::::
Hbo3218         K:::Y:E::::I:::::VE:::::::::::::
NP3152A         :::D:::T:::L:::::TSV::::::::::::
Hut0196         :::PY:K::D:::::::::M::::::::::::
rrnAC0400       N::E::STFS:I:::::Y::D:::::::::::
Hmu1365         N::PA:AI:D::::::::::E:::::::::::