[R] (-) COG2329 Uncharacterized enzyme involved in biosynthesis of extracellular polysaccharides 9 genomes BUD0382 9 proteins a Hsa Halobacterium sp. NRC-1 b Hma Haloarcula marismortui f Hvo Haloferax volcanii g Hbo Halogeometricum borinquense h Hmu Halomicrobium mukohataei i Hut Halorhabdus utahensis j Hla Halorubrum lacusprofundi q Nph Natronomonas pharaonis x Hwa Haloquadratum walsbyi VNG2021 rrnAC3100 HVO_1871 Hbo1748 Hmu2622 Hut1922 hla1076 NP2262A HQ1094A

Pairwise sequence scores
Consensus score	4088	NP2262A	VNG2021	HQ1094A	hla1076	HVO_1871	Hbo1748	Hut1922	rrnAC3100	Hmu2622
2766	NP2262A	4665	2161	2231	2365	2315	2306	2589	2645	2620
2848	VNG2021	2161	3875	2584	2588	2590	2641	2053	2153	2149
2974	HQ1094A	2231	2584	4119	2662	2692	2929	2140	2083	2190
3117	hla1076	2365	2588	2662	4077	2889	2898	2166	2141	2235
3118	HVO_1871	2315	2590	2692	2889	3940	3168	2107	2252	2271
3271	Hbo1748	2306	2641	2929	2898	3168	3986	2235	2287	2348
2719	Hut1922	2589	2053	2140	2166	2107	2235	5456	2948	2959
2767	rrnAC3100	2645	2153	2083	2141	2252	2287	2948	5209	3434
2781	Hmu2622	2620	2149	2190	2235	2271	2348	2959	3434	5231

715 symbols
consensus       --MREAPPTDEGWFALHDFRTVDWDAWRDAPERERDRALEEGVAYLRAHEAVADADEGAS
#oth            143442.3.2...12...2121......2..313.51.2421542.5542322.122.61
NP2262A         MPQ:QP:G:E:::YI:::::SI:::::::::D:::E::::T:TD::Q::VDLK::E::::
VNG2021         :::AD:::::::::V:::CY:::::::::::::D:TA::DDAASF:ADR::L::::::E:
HQ1094A         :::S:V::::::::V:::::::::::::E::THR:EA:VGD::E:::K::SI:::E::S:
hla1076         :::V:::Q:E::::::::::SI:::::::::::::K::I:::K:F:KHR:L:::::::D:
HVO_1871        :::V:::Q:::::::::::::::::::::::D:::R::I:::::::D:::::E::EA:::
Hbo1748         :::TD:::::::::::::::::::::::::::H::N::VG:::E:::T::::E::ED:T:
Hut1922         MVR:R::::L:::Y:::::::I::::::E::Q:V::::ID::RSH:QKAT::::::D:G:
rrnAC3100       MDQ:KP:A:E:::Y::::C:SI::::::E::Q:V:::::S::IGF:D:Y:::E:::::QT
Hmu2622         :MK::P:Q:E:::YV:::L:H:::::::A::::D:::::TD:ID::EYF:::E:SS::Q:

consensus       AVFSVLGHKADLLIVHFRPTLDDLDRAERQFEQTAFAAFTEQATSYVSVTEVSGYT--SR
#oth            111222.21..2223.1.1123311321.3..3.21.41.1144.1...2.2...32144
NP2262A         ::::I::::::FMVL:L:::::H::H:::R::S::L:E::::SS:::::::::::M::HE
VNG2021         G:::IT:Q:::::F::::ES::E:::IQ:A:::::::EY::R:H:::::V:I::::::AP
HQ1094A         :::::::::::I::T:::::::A:S:::::::::T::G::T:P::::::A:I::::::TP
hla1076         GL:::::::::::F::::::::::SSI::R::D::L:N:::RT::::::::::::V:::D
HVO_1871        :::::::::::FVV:::::::::IS:::::::R::L::::::P::::::::::::V:::D
Hbo1748         ::::I::::::::VI:::::::A:S:::::::R::L::Y::::::::::::::::V:::Q
Hut1922         ::YAM:::E:::::::L:::TTAI::LQ:R::::E::G:::RTD:F:::::A:::SKKG:
rrnAC3100       ::YT:M:::::IM:L:L:::MG:::A:::H::::E:::::::EF:::::::A::::EK::
Hmu2622         ::YT:::D::::M:L:L:::MA:::A::::::::E::RY::::::::::::A::::EKA:

consensus       EYF-EGED-DDDEGLRRYIEGKLTPEIPDDEYVCFYPMSKRRGPEYNWYDLPFEERAEHM
#oth            22211232334.4.132.233124.12.233223....1...3132....11121.1231
NP2262A         :LT:D:L:DLE:::M:N:MKQRIY::L::ADH::::::::::::DH:::::::D::R:Y:
VNG2021         D::::DP::SV:D:::Q:F:S:::::::::T::S::::::::Q::Q::::::I:::::M:
HQ1094A         G::::NP::AA:::::Q:MQ::::::::NKD:AG:::::::::ET:::::::ID::::M:
hla1076         :F:::DPE:SV:T::K::::::M::::::::::::::::::::E:::::::S::D::DL:
HVO_1871        D:::::NEE:I:A::L:::::::K:D::E:T:MS:::::::::E:H:::::S:D::RDL:
Hbo1748         D::E::::A:V::::::::::::E:DV:::Q::A:::::::::EK::::::S::::::M:
Hut1922         :FI::::V::::S::A::MRSR:E:::::A:H:S::::D:::D:::::::::::::::::
rrnAC3100       :::::::V::::S::AQ::QAR:H:DV::E:F::::::::::Q:DQ::::TS:::::A:I
Hmu2622         ::::D::V::::S::AQ::QAR:H:D:::E:F::::::::::E:DQ:::::::A:::::I

consensus       SAHGDTGRQYAGKVKQVIASSVGFDDYEWGVTLFADDPTDIKDIVYEMRFDPSSSKYGEF
#oth            45.113.12.12113.2.1111.2.14...1..122.2121.21112....22112112.
NP2262A         :N:::V:::::D::S:I::G:I::::::::I::::::::::::LL:::::::::::FA::
VNG2021         DV:::L:K::::::S::::::::L::M::::::::::L:::::::::::::EV:A:::A:
HQ1094A         E::AE::KE::::I:::::::::::::::::::::::::::::::::::::EV:A:::::
hla1076         AD::EV:KE::::I:::::::::::SH:::::::GS::::::::::::::::A::R::::
HVO_1871        :T:::::::::::I::::::::::::::::::::G::::::::::::::::EV:::::::
Hbo1748         :V::::::E::::I::::::::::::F:::::::SE:::E::N:::D::::DV:::::::
Hut1922         D:::EV::E:::::T:M:TGAI:M::W::::::W:::L::V:ELL::::::::::::AD:
rrnAC3100       KR:::I::G:GSE:S:M::G:I::::W:::I::WS::MRH::ELLT:::::::T:QFA::
Hmu2622         KR:::I::::G:::E:M::G:I::::H:::I::WS::M:HV::LLTQ::::::T::FAD:

consensus       GPFYVGRRFPPADLGAYLAGERVPTDEGDG------------------------------
#oth            .5.21.1213.21.3142313522354565543332232323232232333323223322
NP2262A         ::::I:QQ:E:SE:SSLF:A:PLTSET:H:GA::::::::::::::::::::::::::::
VNG2021         :D:F::::::::::P:FM::::::AP::--::::::::::::::::::::::::::::::
HQ1094A         :T::I:Q:::::::::L:E:KAI::V:Q:VD:::::::::::::::::::::::::::::
hla1076         :E::I::::::E:::::F:::T:::PA:::::::::::::::::::::::::::::::::
HVO_1871        :Q:::::::::S::::F:::DG:::S:FG-::::::::::::::::::::::::::::::
Hbo1748         :Q::I:::::::::::::D::S:::S:HETAH::::::::::::::::::::::::::::
Hut1922         :::W::::ME::::A::M:::TIAG:DEHSAADHSNVPATPEDAADGHGGGEHDAEEHGE
rrnAC3100       :::::::K:D:SE:P:V:::Q:::::DASVPETPADVAEHEHAPADAGASHSHAEEPTAS
Hmu2622         :R:::::::A:S::P:MMN:Q:::SAGANASGATTDGRAQPTDGDHPGTRETATAGQSG:

consensus       ------------DTDAHGEGHG----------HA-----------H---------GDSDH
#oth            212212121111354523353332332121111211111111111211111111121443
NP2262A         :::::::::::::SQTSS:SS:G:::::::::RP:::::::::::S:::::::::T:PS:
VNG2021         ::::::::::::GA::::::ERT::::::::::H::::::::::::::::::::::::::
HQ1094A         ::::::::::::APT::::A::::::::::::::::::::::::::::::::::::::P:
hla1076         ::::::::::::::GDTED:::::::::::::::::::::::::::::::::::::E---
HVO_1871        :::::::::::::ESH::A---::::::::::::::::::::::::::::::::::E---
Hbo1748         ::::::::::::EHG::::A::::::::::::::::::::::::::::::::::::::P:
Hut1922         SAHSGGHPGGHPE::ESAD::PSGHETGEDGS:PTGHETGEDGGHP:::::::::::AGE
rrnAC3100       GAQTGAHAGG:::::S::GT:PGSASEGD::::P:::::::::::::::::::::SEESS
Hmu2622         :::::::::::::AGS::GV:PGSSAEGD::::P::::::::::::DGGDVASRD:EA:E

consensus       DSDD-GGHHGGGG-----------------------------------------------
#oth            545552453443212322122221311222113321233223132231213131321123
NP2262A         :EIAAEDLESRL:RFGV:::::::::::::::::::::DLEEYPAAGYALCFESSADAEA
VNG2021         HDG:D:EQ:HHST:::::::::::::::::::::::::::::::::::::::::::::::
HQ1094A         G:GGG::SSH:QS:::::::::::::::::::::::::::::::::::::::::::::::
hla1076         ----::HD:A:S::::::::::::::::::::::::::::::::::::::::::::::::
HVO_1871        ----::::::E:::::::::::::::::::::::::::::::::::::::::::::::::
Hbo1748         GGS:H::A::::::::::::::::::::::::::::::::::::::::::::::::::::
Hut1922         Q::E:D::P:S::RPSVGDVSYSEVDDLPERLFQLGFRAGEDYEGGEYGLLFYSDADAKE
rrnAC3100       :E::S:SSSSS::RPDVS:ADFEEIDDAAQRLGRLGLHEGDAYDAGDYALVFHSSADAED
Hmu2622         A:ESGD::PD:::RPDPGDASYEETDDLAQRLATMGLSEGEDYDAGDYGLLFYSESDAEA

consensus       --------------------------GGRAAVVSIWTAEDAA-T-----GDHPG--E--G
#oth            33121321232111112113131115316434436334332232121112.533463553
NP2262A         VSDEVDGLRENFDHYDTHVMTTVRAE::ET:AI:L:KN:R::T:ASGFL::L::AA:GV:
VNG2021         ::::::::::::::::::::::::::----------------:-:::::::EA-::-::-
HQ1094A         :::::::::::::::::::::::::P::AS:GG:AHGT::--:-:::::A::SDSRSTTS
hla1076         ::::::::::::::::::::::::::::S:HGDHPHGE:ETSGE:::::::::HSG:EG:
HVO_1871        ::::::::::::::::::::::::::D:HHHHDDGD------:-:::::::::H::-::-
Hbo1748         ::::::::::::::::::::::::::-:H:H-----------:-:::::::S:H::-::-
Hut1922         LADEVDGLRDNFDHYDRHVATLVRAS:::S::::V:::::::E:AAGFL::L::IT:RYA
rrnAC3100       IVDDVSDLESNFDHYDRHVQTAVRADS:QTY:::::::K:::E:AAGFLK:ID:VD:QL:
Hmu2622         LADEVADLRESFDHYDRHVATTVRAQS:::::::::::QE::E:AAGFL::VS:VSDTY:

consensus       --------------GEDGADDA-------DAEIRGELADANIYAGQPHGEDVYATVLYSE
#oth            35111111112221336454544453443253.122.12321...1.2....1.2.1...
NP2262A         APL:::::::::::--::SG::ATHGTDE:D:::::::::D:::::::::::::L:::::
VNG2021         ::::::::::::::--------::::::::DG:::::::ED:::::::::::::::::::
HQ1094A         ::::::::::::::ADTTQS:TSTNTNTQ:S:::EQ::EYD:::::::::::::::::::
hla1076         HG::::::::::::::::D:PS:::::::::D:::::::L:::::K:S::::::::::::
HVO_1871        :::::::::::::::D::DEA:::::::::ED:::Q:E:L:::::K::::::::::::::
Hbo1748         ::::::::::::::::SDE::::::::::SGD:::::E:L::::::::::::::::::::
Hut1922         GVLTADTAEGPAAES:PAQESSEQPAEDSA::::E:::E:GV::::::::::::M:V:::
rrnAC3100       GSL::::::GEGVE:SET:::SDAQAAESSQS::ET:ES:GV::::::::::::L:V:::
Hmu2622         GRL:::::::::::::GA::EPEPTTANSSE::QQQ:::::V:::::N::::F:L:::::

consensus       ADADELFEEVEGLRGNFDHYDTHVKTAVY----------EARDRDRAAVVSIWDTASAAE
#oth            .4412.441123.233.22131.2311..221122222134744425.11.1.12321.2
NP2262A         ::P:::RD:LGA:AEH:ER::::I:S:::D:::::::::AEVGG::T::::L::EKD::D
VNG2021         :G::D::::::::::::E:::::::::::::::::::::DGHEA::R::::::::::::D
HQ1094A         :TT::::S::::::::::::G:::::::::::::::::::::N:H:N::::::E:Q::::
hla1076         ::V::::D::::::::::::G:::::::::::::::::::G:VT::::::::::::::::
HVO_1871        ::::::::::::::::::::P::::::::::::::::::::NE:G:V::::::E::::::
Hbo1748         ::S:::::::::::::::::G::::::::::::::::::::KE:G:S::::::::Q::::
Hut1922         :AP:::AGP:G:::DH:ADAEG::R::::EP::EVGEVTAVE::EAT:I::L:EHEHD::
rrnAC3100       :N:ET:D:::TD::SA:ER:::::Q:T::::::::::::GDT:G:V:::A:L:::ED::Q
Hmu2622         ::PET:ST::TE:TES:::::::EG::::EAGEPLDAATADA:QP::::::L:E:Q:::D

consensus       TAAGFLSELPGVVARAG--EESGFGTMGMFYTVKPEHREDFVETFDTVGGLLAEMDGHRE
#oth            1.123121..2113.2111.11.........211111321.211.13.123.411...43
NP2262A         :::EH::D::DI:::::ED::::::::::::::::DY:::::::::E::::::D::::L:
VNG2021         ::::::AD::E::E:::::::::::::::::ET::::TAE:::K::::A:V::D::::FD
HQ1094A         :::::I::::D::S::::::::::::::::::::S:Y:GE:ND:::::::I:T:::::QQ
hla1076         ::::::::::E::::::::::::::::::::::::::Q:::TD:::D::EI:::::::V:
HVO_1871        :::::::::::I:E::::::G:::::::::::::S:::G::::K:G::::::E:::::FD
Hbo1748         :::::::::::I:S::::::::::::::::::::S::::::::K:::::DV::DT:::Q:
Hut1922         S::DA:A:::::L:::::::GD:::::::::Q:::DY::E::D::GA::EK::::::::G
rrnAC3100       ::SDY:TD:::::G:H::::GD::::::::::::::YH:::::K::::::::K:::::::
Hmu2622         ::S:::TD:::I:G:PD:::GD:::::::::::E:D:::E::::::E::E::D:::::::

consensus       TDLMVNVEDENDMFIASQWDAQEDAMAFFRSDAFRETVQWGRDVLADRPRHVFLA
#oth            .4.14.61122....1.1.32221..2..1.12.31..21..21.111.......
NP2262A         :Q::::ED:::::::::::RDK:A::::::::::::::EY::E::::::::::::
VNG2021         ::::::::NDD::::::::RS:::::::::::D:GD::D::::::::::::::::
HQ1094A         ::::I:L::::::::S:::::R::::S:::::E:S:::E::::::SE::::::::
hla1076         ::::M::::::::::::::H:K:::::::G::E:::::::::E::::::::::::
HVO_1871        ::::::::::D::::::::RS:D:::E::::::::D:::::::::::::::::::
Hbo1748         ::::::I:::D::::::::N::::::E::::::::D:::::::I:::::::::::
Hut1922         :A:L::DD::T::::::N:::R:::::::::ED:::::E:::E::::Q:::::::
rrnAC3100       :S:LF:HD::::::::::::S:K:::::::::D:S:::D::::::::::::::::
Hmu2622         :T:LA:RD:V::::::::::SK::::::::::E:A:::::::T::::::::::::