[EH] (CysH) COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes 9 genomes BUD0068 11 proteins a Hsa Halobacterium sp. NRC-1 b Hma Haloarcula marismortui f Hvo Haloferax volcanii g Hbo Halogeometricum borinquense h Hmu Halomicrobium mukohataei i Hut Halorhabdus utahensis j Hla Halorubrum lacusprofundi q Nph Natronomonas pharaonis x Hwa Haloquadratum walsbyi VNG0459 rrnAC0653 HVO_1078 HVO_1079 Hbo0583 Hbo0584 Hmu0738 Hut1581 hla2226 NP4570A HQ1668A

Pairwise sequence scores
Consensus score	2581	hla2226	HQ1668A	Hbo0584	HVO_1079	Hmu0738	Hut1581	rrnAC0653	NP4570A	VNG0459	HVO_1078	Hbo0583
2216	hla2226	2686	2054	2091	2138	2082	2129	2147	2065	2062	1278	1565
2163	HQ1668A	2054	2555	2113	2132	2037	2028	2079	1958	2021	1280	1546
2240	Hbo0584	2091	2113	2587	2201	2096	2083	2110	2078	2099	1289	1516
2402	HVO_1079	2138	2132	2201	2582	2267	2235	2298	2111	2123	1315	1556
2336	Hmu0738	2082	2037	2096	2267	2565	2268	2320	2069	2082	1285	1537
2283	Hut1581	2129	2028	2083	2235	2268	2587	2336	2022	2067	1288	1511
2370	rrnAC0653	2147	2079	2110	2298	2320	2336	2584	2084	2099	1330	1574
2176	NP4570A	2065	1958	2078	2111	2069	2022	2084	2564	2128	1350	1578
2226	VNG0459	2062	2021	2099	2123	2082	2067	2099	2128	2589	1263	1476
1343	HVO_1078	1278	1280	1289	1315	1285	1288	1330	1350	1263	2517	1742
1631	Hbo0583	1565	1546	1516	1556	1537	1511	1574	1578	1476	1742	2476

337 symbols
consensus       --------------MSEDFPDYLDVDYTDGEGETPADYPSIEDKIEKAIEVTKQGLEQYE
#oth            1111111112223345471.432222142.3313354113354112.1231225212312
hla2226         MQKAYFPSRRHIWR:TDN::ASV::::::::::D::::::LQH:::::::::R::::::D
HQ1668A         :::::::::::::::P:S::E:::::::::NE::ST:::T::S::::::T:::R::::::
Hbo0584         :::::::::::::::P:G:::::::A:E:::::::D:::::H:::::::::::E::::::
HVO_1079        ::::::::::::MSEA:S:::::::::::::::::E::::::::::::::::::::::::
Hmu0738         ::::::::::::::-MAA:::::::::::::::::E:::::NH::::::::::E::::::
Hut1581         ::::::::::::MTSAAE::ASV:::::::D::G:D::::::HQ:::::::::T:::::D
rrnAC0653       ::::::::::::MSE:AE:::::::::::::::D::E::TVNH::::::::::T:::E::
NP4570A         ::::::::::::::-MS:::::V::::::::::::ET::AL:::::::V::VRA:::E::
VNG0459         :::::::::::::::::::::::::::H::D::S::::STLDA:::::::::RD:::S:D
HVO_1078        :::::::::LLYGSHVPRL:RPRLRRRPR:DRRG---L:DAGGQTR:GSDSGRDRARTD:
Hbo0583         ::::::::::::::::PT::SDVTL::DG:RD:DASN:::L::::S::VA::Q:A:D::R

consensus       NPAVMWTGGKDSTLTLYFVKEVAERFDLEVPPVVFIDHYQHFDELHDFVEHWADEWDLDV
#oth            2.21.........12..1131..3441223.321..1.11..11262.134..21.1112
hla2226         ::::::::::::::::::INQ:::K:GY:K:TA:::::F::::DIT:::::::::::I:L
HQ1668A         ::V:::::::::::::::IE:::RQY:::T:ST:::::F:::::I::::DR::N::::::
Hbo0584         ::V:::::::::::C:::I:::::KHGYD:::A:::::::::::I::::DK:::::::::
HVO_1079        ::::::::::::::::::I::::::Y::::::A:::::::::::IM:::::::E::::::
Hmu0738         ::V::::::::::::::::::::D:::::::::::::::::::::IE:::::::::::E:
Hut1581         ::V::::::::::::::::::::D:::::::::::::::::::::I::CK:::E::::EI
rrnAC0653       ::V::::::::::::::::::::::::::::::::::::::::::I::::::::::::::
NP4570A         T::I::::::::::::::::::::E:GYDL::A::::::::::::LE::DR:::::::::
VNG0459         :::I:::::::::::::::::::DQ:::D::ETI::::F::::::::::D::::::::::
HVO_1078        R:::::::::::::V::V:R:::ADTGVP:::::::N:FE::::TEA::RE:::R:R::L
Hbo0583         R:T::::::::::VV::V:R:::AEMGV::::::::::FE::P:TVA:::Q::::::::L

consensus       IWARNEDVGDYVDENGLEPGDDIPVSELSEHNQHHVREILEYEEDTFPFLLDTYVGNHLL
#oth            13...2122411322213...1.1222.31411112132.21.21122111.111.....
hla2226         VY:::::::A::E:H:::::::::::A:N:Q::::I:D::::::::::::::::::::::
HQ1668A         :Y:::N:I:E::::H::::::E::::D::::::::::DL:::::::::::::::::::::
Hbo0584         :Y:::::::A::::HS:S:::::DI::::::::::::NL::::DE:::::::::::::::
HVO_1079        :::::::::A::::::::::::::::::::::::::::::::::::::::::::::::::
Hmu0738         :::::T:I:::::::::D::::::IDA::::::::::::::::::S::::::::::::::
Hut1581         :::::T:::::::::::::::::::DA::::::::I:N::::::::::::::::::::::
rrnAC0653       :::::::::N:::::::::::::::D:::::::::I:N::::::::::::::::::::::
NP4570A         VF:::::I:::::AH::A:::E:::D::D:N:RR:I::::::::::::::::::::::::
VNG0459         VY::::::::::TA:D::::::::I:::DDQ::::::DL::::::S::::::::::::::
HVO_1078        VV::::EFARL----:AS:::E::LAD:GDDTRREL-AR:GH:GE:VVLDA:SFE:::::
Hbo0583         VV:::::FVRL----DAS:::E::LAD:::TTRREL-:R:D::G::LTLDA::FE:::::

consensus       KTVALNDALEEHDVDGIISGVRWDEQEARADETFFSPRHDPDIYPPHDRIQPILQFDEAD
#oth            ....1.121.2312..12..1.....11.11.....2...1131.....112....2.42
hla2226         ::::::N::::Y:I::V:::::::::::::::::::::::::LF::::::::::::A:::
HQ1668A         :::::::T::SYG:::V:::::::::::::K:::::S:::::::::::::::::::::RA
Hbo0584         :::::::::::Y::::V::::::::::::::::::::::::::::::::V:::::::::S
HVO_1079        :::::::T:::::I::V:::::::::::::::::::::::::::::::::::::::E:SA
Hmu0738         ::::::::I:::::::::::I::::::S:::::::::::::::::::::::T:::::::A
Hut1581         ::::::::I::::::::L::::::::::::::::::::::::::::::::::::::A:::
rrnAC0653       :::::::TI::::::::L::I::::::S:::::::::::::::::::::::S::::A:::
NP4570A         ::::::N:::A::I::::::I:::::::::::::::::::::::::::::::::::::G:
VNG0459         ::::::N::::::::::L::I:::::D:::::::::::::::V::::::::::::::::A
HVO_1078        :::::::T::ARGF::VF:::::::::S:G::::::::::SEK::::::VH:::::E:T:
Hbo0583         ::::F::VI:ADGF:::F:::::::::S::::::::A:::SEK::::::VH:::::E:::

consensus       VWDAFWNFVVPDTVEGYPDEGYVPQSDDDLPEGLTQEDVPVSPKYFAGFRSLGSEVSTDK
#oth            1.31..212..121241.22.2..3462...212454.2.1.....2.1....1.2112.
hla2226         ::E::::Y:::::::AF::::::::A:::::::VA:::::I:::::::::::::::::E:
HQ1668A         ::ET::::::::::SA::::::::TGA::::D:VSMN:::::::::::::::::::::E:
Hbo0584         ::Q:::::::::::AE:::D:H::::SE:::ND:::D:I:::::::::::::::::::::
HVO_1079        ::::::Y:A::::::N::ED::::T::T:::::V::::::::::::::::::::::::::
Hmu0738         ::::::::::::::::F:E:::::ET:::::::IE:D:I:::::::::::::::::::::
Hut1581         ::E::::::::::::EF:E::::::GSE:::D:::K::::::::::::::::::::::::
rrnAC0653       ::E::::::::::::::::::::::GQ::::::IEK::::::::::::::::::::::::
NP4570A         ::::::YY:::E:::AF::D::::::Y::::ND:GH::I::::::::::::::::I::E:
VNG0459         ::::::YY:::E::ADF::D:::::DF::::ND::H::I:::::::K:::::::::::::
HVO_1078        ::A:::EYI:::S:::::A-:H::A:I::::D:VEPA:L:::::::E:Y::::T:SGSA:
Hbo0583         I:::::E:I:::AAS::::-:F::E:A::::::IAAT:L:I:::::E::::::T:SGSA:

consensus       SAEEPAWLQDLENTTERAGRAQDKEDLMERLRDLGYM
#oth            2221...3..22311..1..........2........
hla2226         TT::::::::::D::::::::::::::::::::::::
HQ1668A         TT::::::::V::::::::::::::::::::::::::
Hbo0584         TT::::::::M::::::::::::::::::::::::::
HVO_1079        ::::::::::M::::::::::::::::::::::::::
Hmu0738         A:Q::::Q::::G::::::::::::::::::::::::
Hut1581         A:::::::::MA:::::::::::::::::::::::::
rrnAC0653       ::::::::::M::::::::::::::::::::::::::
NP4570A         :D::::::::::::::::::::::::::A::::::::
VNG0459         A::::::::::::::::::::::::::::::::::::
HVO_1078        :DDR:::A:::GASA::E::::::::::G::::::::
Hbo0583         :DDR:::V:::GAS::::::::::::::G::::::::