[E] (AsnB) COG0367 Asparagine synthase (glutamine-hydrolyzing) 9 genomes BUD0254 9 proteins a Hsa Halobacterium sp. NRC-1 b Hma Haloarcula marismortui f Hvo Haloferax volcanii g Hbo Halogeometricum borinquense h Hmu Halomicrobium mukohataei i Hut Halorhabdus utahensis j Hla Halorubrum lacusprofundi q Nph Natronomonas pharaonis x Hwa Haloquadratum walsbyi VNG0867 rrnAC1359 HVO_1050 Hbo0545 Hmu1879 Hut1421 hla2686 NP2978A HQ1691A

Pairwise sequence scores
Consensus score	2679	HQ1691A	NP2978A	hla2686	VNG0867	HVO_1050	Hbo0545	Hut1421	Hmu1879	rrnAC1359
1607	HQ1691A	3260	1393	1404	1350	1657	1569	1297	1449	1361
1887	NP2978A	1393	2726	1415	1516	1611	1561	1548	1547	1668
1794	hla2686	1404	1415	2911	1432	1673	1610	1520	1512	1474
1892	VNG0867	1350	1516	1432	2704	1633	1528	1582	1486	1664
2132	HVO_1050	1657	1611	1673	1633	2718	2042	1616	1685	1732
2033	Hbo0545	1569	1561	1610	1528	2042	2675	1590	1645	1679
1976	Hut1421	1297	1548	1520	1582	1616	1590	2794	1617	1734
1961	Hmu1879	1449	1547	1512	1486	1685	1645	1617	2730	1770
2029	rrnAC1359	1361	1668	1474	1664	1732	1679	1734	1770	2697

452 symbols
consensus       ----------------------LRGADPDTVRDAIESGDPLPGTRGFAGEL-DG------
#oth            111111112222223222231421.124341431245522.2.23....31222221111
HQ1691A         MMSGPPTNPPGQPQSSTKNESI::::STE::H:::SDTIA:A::G::::YI:::::::::
NP2978A         ::::::::::::::::::::::MQ:TAVEA::ECL:TKA::A::C:::::::::::::::
hla2686         ::::::::::::::MTTPSSSD::::S::RIC::LRG::::::G:::::L:R:PPNREGP
VNG0867         ::::::::::::::::::::::M:::A::A:L::VQ::::::::G::::::P::::::::
HVO_1050        ::::::::::::::::::::::M:::S::L::R:LAER::::::A:::::::::::::::
Hbo0545         :::::::::::::::::::::M:::::ET:::A::::S:::S::A:::::::N:::::::
Hut1421         ::::::::VTMVSVPSDPSPSR:Q:::::V::A:::A::::::::::::R::::::::::
Hmu1879         :::::::::::::::::::::MI::::ASL::R::RDR:S:::::::::R::SAEGR::P
rrnAC1359       ::::::::::::::::::::::MQ:::A:::A::LA:D:A:::AG:::::::::::::::

consensus       RLVRDVLGRQPLFVERA-----------DPGAWSFDPTDLADPVPVPAGHVRTADG----
#oth            411......41314135432333111124457.122235.42.452.1.44356442132
HQ1691A         M::::::::::I:L:T:TQSSHG:::::VMPD:A:N:LS:EA:ATL:P:A:YDPSQRAIS
NP2978A         GV:::::::Y:A:T:PE:::::::::::T:S:::P:::::SE:E:::::CI:::::::::
hla2686         V::::::::E::::::GGADPASGGDPT:S:I::::R:::D::E:::::T:ASSA:::::
VNG0867         ::A::::::RLCY:---::::::::::::GDE::H::R:::A:TLL::::::APGA::TG
HVO_1050        :::::::::::::S::::::::::::::::AK::::HR::D:::A::::RA:QTA:EAAG
Hbo0545         T::::::::::::S::N:::::::::::::ET:A::RRE:::::R::::TR::D::::::
Hut1421         :::::::::Y::::::D:::::::::::N:DS:::E:GK::::::::::T:QDGET::::
Hmu1879         T::::::::V:::A::GAADPT::::::AD:::A::::T:G:::S:::::C::::E::::
rrnAC1359       T::::::::Y:::::::VSDEAR::::L:::Q::H:::A::::RSF::::I:NES:::::

consensus       -DRRV----------------------------------WSLPDPAP---TDRDAAVDAV
#oth            245431111111111111111111111111111111111.1..53431156243313441
HQ1691A         D:DIIYMDDNTSIDDANSDQQGNTVDVDVEIRHLDIARY::::K:PV::T::N:E:YRR:
NP2978A         :::Q:::::::::::::::::::::::::::::::::::::::::E:::VA:T:R::S::
hla2686         :AE:::::::::::::::::::::::::::::::::::::::::EE:VDAESGQ::::EA
VNG0867         SG:P:::::::::::::::::::::::::::::::::::::::E::::::-TG:EP:ER:
HVO_1050        D:E::::::::::::::::::::::::::::::::::::::::::PA::A::::::L:R:
Hbo0545         ::K::::::::::::::::::::::::::::::::::::::::T:::::E:::E::LAN:
Hut1421         :VTQW::::::::::::::::::::::::::::::::::::::Q:S::::RSGRR:::::
Hmu1879         ::DAR:::::::::::::::::::::::::::::::::::T::EGDR:::S:D:T::A::
rrnAC1359       :TQQR::::::::::::::::::::::::::::::::::::::SLD:::FG::ET:IET:

consensus       RDAVDTAID---AVDT-DGLAVAFSGGVDSALLAAGL-DAPLYVAGFEGSHDIAAAREAA
#oth            55127333411142453532.1.....1..212..22112.1..2.13211.13..1211
HQ1691A         KK:II:QTQKQTTDNSNKST:I:::::I:::VV:::RPN::C:::::D:C:::T::Q::L
NP2978A         A::I:A::::::S::-:GAP:::::::I::S::::::::G::::G::::::::T:::S::
hla2686         LG::LGE:K:::TEANRND::::::::I::GVV::AVP:::C:::::::C:::E::::::
VNG0867         ::::AG:LG:::G:PAG:D:::::::::::::A::A::::::::V:YAD:S:::::::S:
HVO_1050        ::::FESVR:::S::D:::::::::::::::VV:::VP:::C::::::::::V:::::::
Hbo0545         ES::HG:VQ:::N:ES:E:::::::::::::VV:::VP:::C::::::::::V::::D::
Hut1421         :S:L:::L::::::E::::::::::::::::::::R:::V:::::::PE:::VR::::::
Hmu1879         :R:IR::T:::::::::AST:::::::::::::::R:::::::::::::::::::::S::
rrnAC1359       GET:E:S:::::::::::S::I:::::::::::::R:::::::::::PD::::E:::S::

consensus       AAMDR--DLRVVELTHDDLERAVPEVVAATGRTNPMDVQIALPLYLVAERVAADGFDRLA
#oth            4112322212.2.321321331215213121.211.1221211.114.421.3..31..1
HQ1691A         S::::NA::H:I:IS:T:IIE:I:QL:S:I:::::::LA:TV::FI::R:::::::::::
NP2978A         DLL::::RV::::F::A:::::T:T::E::::S::::::L::S:::I::A::E::H::::
hla2686         T::EL::::::::I:::::V:::GA:A:::::R:::::A::V::::A::AA:T::I::::
VNG0867         :::G:AD::::H:V:LA:I:A:::T:AR:I::::A:A:::::::F:::A:A::::YS::V
HVO_1050        :::::::::T::::::EA:::::::I:::L:::T::::::V:::::L::::::::Y::::
Hbo0545         D::::::E:H:::::::A:RE::::L:SVL:::::::I::V:::::::KQ::::::::::
Hut1421         :LL:A:::::::::D:AL:::T::D:::::::::A:::E::V::::::S:A:T:::::::
Hmu1879         ::L::::E:T:::F:::::VS:::RIAR::::::A:::::::::F:T::::::::Y:::V
rrnAC1359       ELLGT::EV::::::::AI::::::IAR::A:::A:::::::::::::::::G:::::::

consensus       VGQGADELFGGYAKVAKAPDDPRVEADTVRGARREVVATLPDQLERDVLALRAAGVEPVA
#oth            1...........1..3111413.24.2.1...31.42611.4.11..3122.11.11.11
HQ1691A         L::::::::::::::Q:::T::::N:T:I:::Q::LLN:V:K:A:::M::I::G::::IT
NP2978A         L::::::::::::::::::A::::D::::::::::::GS::S::::::::V::::I:::T
hla2686         ::::::::::::S::VNPAG:D::A:E::::::T:T:R:::E::D:::::::::::::::
VNG0867         :::::::::::::::::::T:::TD::::::::::T:QS::A:::::LR::::::::::T
HVO_1050        :::::::::::::::Q::::::::::::::::A::MIL:::::::::L:V::::::D:::
Hbo0545         :::::::::::::::E::::::::::::::::AT:M:R:::E:::::::T::::::::::
Hut1421         ::::::::::::::::N::EHS::G:::::::H::GLE:::::::::I::I:::::::::
Hmu1879         ::::::::::::::::N::::H:I::::::::Q:::I::::E::::::::::G:::::::
rrnAC1359       L::::::::::::::::::E::::::::::::Q:::I:S::::::::::::::::::::T

consensus       PLLHDRVVEAALALPGELLVSGDT-RKVALRRAA----REWLPDSVADRDKKAVQYGTYA
#oth            .1.3.42142..5.2233.142352..4.1.41111114461.2411512...2...112
HQ1691A         :F:::SI::S::T::ES:::C::Q:::I:::M::::::DGIV:E::RTAS:::::::::V
NP2978A         :::C:G::S:::R::A::::A:::::::::::::ETVPDGGV:ADIR::E:::L:::SLL
hla2686         :::D::L:A::::::::::A:D:E::::::::::::::TDRV:E::RTA::::::::::V
VNG0867         :::::D::R::::::::W:::::::::R:::E:::::::S::::A::::::::::::SLV
HVO_1050        ::::::::S:::P::E:F::D::R:::::::E::::::SGV::E:::EA:::::::::::
Hbo0545         :::::D::S:::P:::::::ADSR:::::::EV:::::G:LV:::::SA:::::::::::
Hut1421         :::D:H::::::S:::R::::PRGD::Y:F:L:T:::::A::::RI:F:E:::L:::SLV
Hmu1879         :::S:G:::T::G:V::Q::R:EDE::W::::::::::S::::EEI:R::::::::::::
rrnAC1359       :::::::IG:::R:D:D:::D:E::::::::A::::::::S:::EI:::::::A:::SL:

consensus       ARELDRLARQAGFKRRMDNHVTQYVESLIE--
#oth            1..3.....11.1....12.231.23214522
HQ1691A         S:::::::::D:::::::H:IQK::H:::K::
NP2978A         :::F::::::::::::::::LGK:I:DRCQDA
hla2686         S::I:::::R::::::::D::G::LDA:LAEQ
VNG0867         :::V::::::::::::::D:::::::AR::E:
HVO_1050        S::::::::::::::::E:::Q::::::V:::
Hbo0545         ::::::::::::Y::::E:::E:::Q::::E:
Hut1421         ::::::::::::::::::::::::::::KG::
Hmu1879         :::::::::RD:::::::D:::K:::::L-::
rrnAC1359       ::::::::::::Y:::::D:::::::::V:::