Hlac_2868
Gene Hlac_2868 in replicon pHL500
Hlac_2868 codes for a family 42 beta-galactosidase enzyme. It was first investigated using bioinformatics to compare it to homologous haloarchaeal proteins. See DasSarma et al. Plos One. 2013 Mar;8(3):e58587. The enzyme was then cloned, overexpressed, purified, and characterized using NRC-1 as a haloarchaeal expression system. See Karan et al. BMC Biotech. 2013 Jan;13(3). In order to determine the source of the enzyme’s cold adaptation the bioinformatic and genetic approaches were combined to perform site directed single amino acid mutations and it was determined that single amino acid changes can affect the cold adaptation of the enzyme. See Laye et al. Proc Natl Acad Sci U S A. 2017 Oct;114(47):12530-5. Function of the enzyme with Martian perchlorate concentration was also tested. See Laye and DasSarma Astrobiology. 2017 Nov 30. doi: 10.1089/ast.2017.1766.
- More about this gene
- Blast DNA databases using NCBI Blast
- Blast protein databases using NCBI Blast
- KEGG Enzyme 3.2.1.23
- KEGG Gene
- Get protein data of this gene from GenBank
- See protein structure at GTOP
- Get HOG data of this gene from local HOG database: aHOG3941
- Get COG data from NCBI's COG database: COG1874G
- Get BUD data for this gene from our local BUD database: BUD3941
- Retrieve flanking sequence
| Gene ID | Name | Size (bp) | Annotation |
|---|---|---|---|
| 2865 | Hlac_2865 | 1122 | ABC transporter related |
| 2866 | Hlac_2866 | 1149 | Mandelate racemase/muconate lactonizing protein |
| 2867 | Hlac_2867 | 819 | short-chain dehydrogenase/reductase SDR |
| 2868 | Hlac_2868 | 2103 | Beta Galactosidase Family 42 |
| 2869 | Hlac_2869 | 2040 | Alpha-galactosidase-like protein |
| 2870 | Hlac_2870 | 957 | PfkB domain protein |
| 2871 | Hlac_2871 | 432 | transposase IS200-family protein |